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Comparing low-pass sequencing and genotyping for trait mapping in pharmacogenetics

May 30, 2021 Timmothy Mckinney A powerful tool for genome analysis in maize: development and evaluation of the high density 600 k SNP genotyping array., Bacteria, Blocking, blocking peptide, blood, Blot, Camel, Cultrex, Default, Dot, EIA, electrophoresis, elisa, extract, Hamster, High-density 80 K SNP array is a powerful tool for genotyping G. hirsutum accessions and genome analysis., Horse, Multiple Locus Variable number of tandem repeat Analysis: A molecular genotyping tool for Paenibacillus larvae., Pig, Pigeon, Plant, plasmid, Plate, Rabbit, Rat, Sheep, Yeast 0

Comparing low-pass sequencing and genotyping for trait mapping in pharmacogenetics
Low move sequencing has been proposed as an economical different to genotyping arrays to determine genetic variants that affect multifactorial traits in people. For frequent ailments this sometimes has required each massive pattern sizes and complete variant discovery. Genotyping arrays are additionally routinely used to carry out pharmacogenetic (PGx) experiments the place pattern sizes are prone to be considerably smaller, however clinically related impact sizes prone to be bigger. To assess how low move sequencing would examine to array primarily based genotyping for PGx we in contrast a low-pass assay (in which 1x protection or much less of a goal genome is sequenced) together with software program for genotype imputation to straightforward approaches.
We sequenced 79 people to 1x genome protection and genotyped the identical samples on the Affymetrix Axiom Biobank Precision Medicine Research Array (PMRA). We then down-sampled the sequencing information to 0.8x, 0.6x, and 0.4x protection, and carried out imputation. Both the genotype information and the sequencing information had been additional used to impute human leukocyte antigen (HLA) genotypes for all samples. We in contrast the sequencing information and the genotyping array information in phrases of 4 metrics: total concordance, concordance at single nucleotide polymorphisms in pharmacogenetics-related genes, concordance in imputed HLA genotypes, and imputation r2.
Overall concordance between the 2 assays ranged from 98.2% (for 0.4x protection sequencing) to 99.2% (for 1x protection sequencing), with qualitatively related numbers for the subsets of variants most vital in pharmacogenetics. At frequent single nucleotide polymorphisms (SNPs), the imply imputation r2 from the genotyping array was 0.90, which was corresponding to the imputation r2 from 0.4x protection sequencing, whereas the imply imputation r2 from 1x sequencing information was 0.96.  These outcomes point out that low-pass sequencing to a depth above 0.4x protection attains increased energy for affiliation research when in comparison with the PMRA and ought to be thought-about as a aggressive different to genotyping arrays for trait mapping in pharmacogenetics.

Rodent management programmes can combine Echinococcus multilocularis surveillance by facilitating parasite genotyping: the case of Arvicola terrestris voles screening in France

The tapeworm Echinococcus multilocularis is the causative agent of alveolar echinococcosis, essentially the most severe parasitic illness for people in Europe. In Europe, the E. multilocularis lifecycle is predicated on a prey-predator relationship between the purple fox and small rodents. Over the final three many years, the surveillance of E. multilocularis an infection in purple foxes has led to the outline of a wider distribution sample throughout Europe. France constitutes the present European western border, however solely the north-eastern half of the nation is taken into account endemic. The purple fox is the host primarily focused in E. multilocularis surveillance programmes, however surveys focusing on small rodents could also be helpful for acquiring molecular information, particularly when the time-consuming trapping is already carried out in devoted pest-control programmes.

Here, we screened for parasitic lesions in the livers of 1238 Arvicola terrestris voles originating from the historic, however uncared for focal space positioned in central France (Auvergne area) and from Hautes-Alpes, a just lately recognized endemic division in south-eastern France. This screening recognized six voles contaminated with E. multilocularis in Hautes-Alpes and none in Puy-de-Dôme (Auvergne area) after molecular affirmation. The absence of contaminated rodents from Puy-de-Dôme may be primarily defined by the commonly low prevalence reported in intermediate hosts. The contaminated Hautes-Alpes samples come all from the identical trapping website located at round 5 km from one of many three fox faecal samples with E. multilocularis DNA collected 15 years prior, thereby confirming the existence and persistence of the E. multilocularis lifecycle in the world.

All the rodent E. multilocularis samples from Hautes-Alpes confirmed the identical EmsB microsatellite marker profile. This profile has beforehand been described in Europe solely in the Jura division (central jap France), positioned not less than 180 km additional north. Successive migrations of contaminated foxes from the historic focal space, together with from Jura, to Hautes-Alpes could clarify the detection of the parasite in A. terrestris in Hautes-Alpes. Existing trapping efforts in areas the place farmers entice A. terrestris for surveillance and pest management may be an efficient complement to sampling foxes or fox faeces to acquire E. multilocularis molecular profiles.

Comparing low-pass sequencing and genotyping for trait mapping in pharmacogenetics

Chemotherapy-associated clonal hematopoiesis mutations ought to be taken severely in plasma cell-free DNA RAS/BRAF genotyping for metastatic colorectal most cancers

 Genotyping of plasma cell-free DNA (cfDNA) is an more and more vital methodology to evaluate the tumor mutation standing in colorectal most cancers (CRC) sufferers. Clonal hematopoiesis (CH) releases non-tumor somatic mutations into blood, inflicting false optimistic outcomes in cfDNA-based tumor genotyping. It continues to be not clear if CH ought to be examined in all CRC sufferers present process cfDNA evaluation. We analyzed cfDNA KRAS/NRAS/BRAF genotypes in 236 metastatic CRC sufferers, who had matched tissue genotyping outcomes, by next-generation sequencing utilizing plasma cfDNA.
Three CH-derived mutations, KRAS Q61H, KRAS G12D and KRAS G12V, had been recognized in the affected person cohort. All three sufferers harboring corresponding CH-derived mutations had a previous chemotherapy historical past. The CH-derived KRAS G12V mutation in a affected person was discovered solely current in lymphocytes and persisting below remedy. For all cfDNA mutations, the CH-derived ones had been clustered in the sufferers with <5% mutation AF and prior chemotherapy.

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The prevalence of CH in CRC sufferers was restricted, and prior chemotherapy was a contributing issue of CH. It is really helpful for sufferers with <5% mutation AF and prior chemotherapy to have genotyping evaluation of their PBCs following plasma cfDNA genotyping. The cfDNA-only mutations with allele frequencies (AFs) <5% had been extremely suspicious for being CH-derived mutations. The origins of cfDNA mutations had been confirmed by droplet digital polymerase chain response (ddPCR) utilizing paired peripheral blood cells (PBCs) and CH-derived mutations had been lastly decided. One affected person with a CH-derived mutation was adopted up and the subpopulation of blood cells, in which CH was current, was investigated.
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A PCR-RFLP method for genotyping of inversion 2Rc in Anopheles coluzziiPrevious

A PCR-RFLP method for genotyping of inversion 2Rc in Anopheles coluzzii

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QED Bioscience develops novel monoclonal and polyclonal antibody products for our academic, biotechnology, diagnostic and pharmaceutical research clients.

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QEDBioscienceQED Bioscience@QEDBioscience·
17 Jul 2019

In Search of a Cure for the Common Cold — https://www.qedbio.com/blog/in-search-of-a-cure-for-the-common-cold/

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23 May 2019

Alpha-1-Antitrypsin Deficiency and Autoimmune Disease - https://www.qedbio.com/blog/alpha-1-antitrypsin-deficiency-and-autoimmune-disease/

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QEDBioscienceQED Bioscience@QEDBioscience·
23 May 2019

Adenosine deaminase Severe Combined Immunodeficiency (ADA-SCID) - https://www.qedbio.com/blog/adenosine-deaminase-severe-combined-immunodeficiency-ada-scid/

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QEDBioscienceQED Bioscience@QEDBioscience·
23 May 2019

We Grow Our Own - https://www.qedbio.com/blog/we-grow-our-own/

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QEDBioscienceQED Bioscience@QEDBioscience·
23 May 2019

DNA Mismatch Repair - https://www.qedbio.com/blog/dna-mismatch-repair/

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Categories
  • A powerful tool for genome analysis in maize: development and evaluation of the high density 600 k SNP genotyping array.
  • Bacteria
  • Blocking
  • blocking peptide
  • Blog
  • blood
  • Blot
  • Camel
  • Cultrex
  • Default
  • Dot
  • EIA
  • electrophoresis
  • elisa
  • extract
  • Hamster
  • High-density 80 K SNP array is a powerful tool for genotyping G. hirsutum accessions and genome analysis.
  • Horse
  • Multiple Locus Variable number of tandem repeat Analysis: A molecular genotyping tool for Paenibacillus larvae.
  • Pig
  • Pigeon
  • Plant
  • plasmid
  • Plate
  • Rabbit
  • Rat
  • Sheep
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